Pulse sequences developed in the Veglia laboratory for solution and solid-state NMR

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Please cite the respective references when you use these sequences

 

The pulse sequences are available for download below:

All pulse sequences have been optimized for VNMRS Spectrometers

 

Adiabatic R1rho and R2rho Pulse Sequences

Pulse sequences used to measure 15N R1rho and R2rho relaxation rates using a train of adiabatic pulses. Used to probe microsecond to millisecond dynamics in biomolecules.

1. Varian Version (VNMRJ 2.2C)

2. Bruker Version (Topspin 2.1)
Coded by Dr. Youlin Xia, NMR Facility, University of Minensota Twin Cities

Citation: Mangia S*, Traaseth NJ*, Veglia G, Garwood M, Michaeli S (2010) Probing Slow Protein Dynamics by Adiabatic R1rho and R2rho NMR Experiments. J Am Chem Soc 132, 9979-9981.

 

Sensitivity Enhanced Separated Local Field Sequences

Pulse sequences for oriented solid-state NMR used to enhance the sensitivity/resolution of 2D PISEMA, SAMPI4, and HIMSELF experiments that correlate 15N anisotropic chemical shifts with 1H-15N dipolar couplings.

1. Sensitivity-Enhanced PISEMA
Citation: Gopinath T, Veglia G (2009) Sensitivity Enhancement in Solid-State NMR Experiments via Single- and Multiple-Quantum Dioplar Coherences. J Am Chem Soc 131, 5754-5756.

2. Sensitivity-Enhanced SAMPI4
Citation: Gopinath T, Verardi R, Traaseth NJ, Veglia G (2010) Sensitivity Enhancement of Separated Local Field Experiments: Application to Membrane Proteins. J Phys Chem B 114, 5089-5095.

3. Sensitivity-Enhances HIMSELF
Citation: Gopinath T, Verardi R, Traaseth NJ, Veglia G (2010) Sensitivity Enhancement of Separated Local Field Experiments: Application to Membrane Proteins. J Phys Chem B 114, 5089-5095.

4. Sensitivity-Enhanced Constant-Time PISEMA
Citation: Gopinath, T, Veglia, G (2010)  Improved Resolution in Dipolar NMR Spectra Using Constant Time Evolution PISEMA Experiment. Chem. Phys. Lett., 494, 1-3, 104-110.

 

Sensitivity Enhanced HETCOR Sequences
Pulse sequences for oriented solid-state NMR used to enhance the sensitivity of 2D and 3D heteronuclear correlation (HETCOR) experiments that correlate 15N anisotropic chemical shifts with 1H anisotropic chemical shifts.

1. Sensitivity-Enhanced HETCOR
Citation: Gopinath T, Traaseth NJ, Mote K, Veglia G (2010) Sensitivity Enhanced Heteronuclear Correlation Spectroscopy in Multi-Dimensional Solid-State NMR of Oriented Systems via Chemical Shift Coherences. J Am Chem Soc 132, 5357-5363.

2. Sensitivity-Enhanced PISEMAI-HETCOR
Citation: Gopinath T, Traaseth NJ, Mote K, Veglia G (2010) Sensitivity Enhanced Heteronuclear Correlation Spectroscopy in Multi-Dimensional Solid-State NMR of Oriented Systems via Chemical Shift Coherences. J Am Chem Soc 132, 5357-5363.

 

Magic Angle Spinning Solid State NMR Sequences

1. Frequency Selective Dipolar Recoupling
Citation: Traaseth, NJ, Veglia, G (2011) Frequency-Selective Heteronuclear Dephasing and Selective Carbonyl Labeling to Deconvolute Crowded Spectra of Membrane Proteins by Magic Angle Spinning NMR J. Mag. Res. doi:10.1016/j.jmr.2011.03.013